نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار بخش کشاورزی دانشگاه پیام نور ایران
2 استادیار بخش کشاورزی، دانشگاه پیام نور، ایران
چکیده
شناسایی مکانیسم مولکولی-پروتئینی زعفران در مقابل تنش اسمزی در ایران که یکی از کشورهای خشک و نیمهخشک جهان محسوب میشود، از اهمیت ویژهای برخوردار است. بدین منظور و برای بررسی پاسخ پروتئینی به تنش اسمزی اعمال شده با غلظتهای مختلف PEG6000، آزمایشی با استفاده از دو توده زعفران (تربتحیدریه و مرند) به روش کشت آبکشت (هیدروپونیک) در مرحلة رشد رویشی (گیاهچه‌ای) در گلخانه انجام شد. دو هفته پس از اعمال تنش اسمزی نمونهبرداری از کلیه واحدهای آزمایشی انجام شد. بر طبق نتایج بدست آمده از لحاظ واکنش پروتئینی تحت تنش اسمزی توده تربتحیدریه نسبت به توده مرند در وضعیت مطلوبتری قرار داشت. همچنین نتایج تجزیه پروتئوم دو توده نشان داد که از بین 17 لکه پروتئینی معنیدار بین گیاهان شاهد و تیمارهای تنش اسمزی، تعداد چهار لکه پروتئینی بین دو توده مشترک و تعداد شش و هفت لکه پروتئینی بترتیب منحصر به توده تربتحیدریه و مرند بودند. در تفسیر پاسخ اختصاصی میتوان اظهار داشت که توده متحملتر تربتحیدریه تحت تنش بیشتر در مسیر دفاع آنتیاکسیدانی فعالیت داشته و شامل افزایش فعالیت پروتئینهای harpin binding protein 1 و peroxiredoxin بودند. در حالی که توده حساستر مرند بیشتر آسیب خود را در مسیرهای مختلف بیولوژی از طریق کاهش فعالیت پروتئینها خصوصاً کاهش پروتئین Thylakoid lumenal 29.8 kDa دخیل در واکتش نوری فتوسنتز داشت. در مجموع پاسخ پروتئینی متفاوت دو توده و مهمترین واکنش پروتئینی و مسیرهای متابولیکی مهم تودههای مورد مطالعه زعفران مشخص شد.
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