نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه تربت حیدریه، تربت حیدریه، ایران.

2 دانشجوی کارشناسی ارشد، گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه تربت حیدریه، تربت حیدریه، ایران.

3 دانشیار، گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران.

چکیده

در این پژوهش، خواص ضد‌میکروبی سافرانال زعفران و کپسوله‌سازی آن با β-سیکلودکسترین (β-CD) به‌عنوان دیواره و صمغ عربی (GA) به‌عنوان امولسیفایر با روش رسوب همزمان بررسی شد. تأثیر صمغ عربی بر میزان کپسوله‌سازی، اندازه ذرات، سرعت رهاسازی و ویژگی‌های ساختاری میکروکپسول‌ها ارزیابی گردید. در نسبت بهینه سافرانال به صمغ عربی(1:3 w/w)، میزان کپسوله‌سازی ۶۱/۸۷ درصد و اندازه متوسط ذرات ۲۰/۸ میکرومتر به دست آمد. تحلیل FTIR نشان داد که صمغ عربی تنها نقش امولسیفایر دارد و بر ساختار شیمیایی β-CD تأثیر نمی‌گذارد. بررسی DLS توزیع یکنواخت ذرات و XRD حفظ ساختار بلوری سافرانال را تأیید کرد. فعالیت ضد‌میکروبی سافرانال و میکروکپسول‌های آن علیه Botryodiplodia theobromae وColletotrichum gloeosporioides  بررسی شد و نتایج نشان داد که کپسوله‌سازی موجب بهبود ظرفیت بارگذاری و راندمان کپسوله‌سازی می‌شود. این پژوهش نشان داد که استفاده از β-CD و صمغ عربی می‌تواند موجب تثبیت و حفظ خواص ضد‌میکروبی سافرانال شود و رهاسازی را کنترل کند. این ویژگی‌ها، کپسوله‌سازی سافرانال را برای کاربرد در صنایع دارویی و غذایی مناسب می‌سازد.

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