Document Type : Original Article

Authors

Abstract

Saffron (Crocus sativus L.) is one of the most valuable medicinal cash crops that mainly cultivated in arid and semi-arid areas. In order to study the effect of planting density on flower quality in Iranian and Spanish corms of saffron, an experiment was conducted as factorial layout based on a randomized complete block design with three replications during two growing seasons (2013-2014 and 2014-2015). Two corm types (a1: Iranian and a2: Spanish corms) and three planting densities (d1: 40, d2: 48 and d3: 60 corms.m-2) were considered as treatments. The results showed that the effect of different planting density on crocin, picrocrocin and safranal contents were significant (p≤0.01). The highest numbers of crocin (202.01), picrocrocin (72.23) and safranal (36.30) contents were obtained in 40 corms.m-2, moreover the highest number of crocin (200.73), picrocrocin (71.24) and safranal (28.91) contents were obtained in Spanish corm. However, the highest numbers of flower (27.21 flowers.m-2), flower fresh weight (13.58 g.m-2) and dry weight (stigma+style) (0.144 g.m-2) were obtained from Iranian corms. Spanish corm in comparison with Iranian corm was produced more crocin, picrocrocin and safranal contents. In contrast, Iranian corms produced the highest saffron dry weight.
Saffron (Crocus sativus L.) is one of the most valuable medicinal cash crops that cultivated mainly in arid and semi-arid areas. In order to study the Effect of planting density on flower quality in Iranian and Spanish Saffron (Crocus sativus L.), an experiment was conducted in factorial with randomized complete block design with three replications in two growing seasons (2013- 14 and 2014- 15). The saffron corm type considered in two levels (a1: Iranian corm and a2: Spanish corm) and the planting density were in three levels (d1:40, d2:48, d3:60 corm.m-2). Results showed that the effect of different planting density and saffron corms type on number of flowers, flower fresh and dry weight (stigma + style) were significant. In addition the effect of different planting density on crocin, picrocrocin and safranal were significant (p<0.01). The highest number of crocin (202.01), picrocrocin (72.23) and safranal (36.30) were obtained in 40 corm.m-2, moreover the highest number of crocin (200.73), picrocrocin (71.24) and safranal (28.91) were obtained in Spanish corms. However, the highest numbers of flower (27.21 flower.m-2), flower fresh weight (13.58 g.m-2) and saffron (stigma+ style) dry weight (0.144 g.m-2) were obtained from Iranian corms. While, Spanish corms in comparison with Iranian corms were produced more crosin, picrocrosin and saffranal. In contrast, Iranian corms produced the highest saffron dry weight.

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Abdullaev, F.I., and Espinosa-Aguirre, J.J., 2004. Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect. Prev. 28, 426–432.
Abdullaev, F.I., 2002. Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativus L.). Exp. Biol. Med. 227, 20–25.
Afghanistan Statistical Year Book., 2016. Ministry of Agriculture, Irrigation and Livestock.
Agayev, Y.M., Fernandez, J.A., and Zarifi, E., 2009. Clonal selection of saffron (Crocus sativus L.): The first optimistic experimental results. Euphytica. 169, 81-99.
Behdani, M.A., Koocheki, A., Nassiri Mahallati, M., and Rezvani Moghaddam, P., 2006. Evaluation of quantitative relationships between saffron yield and nutrition (on farm trial). Iran. J. Field Crop Res. 3, 1–14. [in Persian with English Summary].
Beheshti, A., and Faravani, M., 2003. Investigation the effect of different planting proportions and densities on yield and yield components of saffron and caraway mixed cropping. 3rd National Congress of Saffron, Mashhad, Iran, 2-3 December. [in Persian with English Summary].
Bhandari, P.R., 2015. Crocus sativus L. (saffron) for cancer chemoprevention: A mini review. J. Trad. Compl. Med. 5, 81-87.
Carmona, M., Zalacain, A., Sanchez, A.M., Novella, J.L., and Alonso, G.L., 2006. Crocetin esters, picrocrocin and its related compounds present in Crocus sativus stigmas and Gardenia jasminoides fruits. Tentative identification of seven new compounds by LC-ESI-MS. J. Agric. Food Chem. 54, 973–979.
Ghorbani, M., 2007.The economics of saffron in Iran. Acta Hort. 739, 321–331.
Gresta, F., Avola, G., Lombardo, G.M., Siracusa, L., and Ruberto, G., 2009. Analysis of flowering, stigmas yield and qualitative traits of saffron (Crocus sativus L.) as affected by environmental conditions. Sci. Hort. 119, 320–324.
Gresta, F., Lombardo, G.M., Siracusa, L., and Ruberto, G., 2008a. Effect of mother corm dimension and sowing time on stigma yield, daughter corms and qualitative aspects of saffron (Crocus sativus L.) in a Mediterranean environment. J. Sci. Food Agric. 88, 1144–1150.
Gresta, F., Lombardo, G.M., Siracusa, L., and Ruberto, G., 2008b. Saffron, an alternative crop for sustainable agricultural systems. A review. Agron. Sustain. Dev. 28, 95–112.
ISO., 2003. Saffron (Crocus sativus L.) Part I: Specifications, and Part II: Test Methods. International Standards Organization, Geneva, Switzerland, ISO 3632-1/2.
Kafi, M., Rashed Mohassel, M.H., Koocheki, A., and Mollafilabi, A., 2002. Saffron, Production and Processing. Ferdowsi University of Mashhad Press, Mashhad, Iran. 276 pp. [in Persian].
Koocheki, A., Jahani, M., Tabrizi, L., and Mohammad Abadi, A.A., 2011. Investigation on the effect of biofertilizer, chemical fertilizer and plant density on yield and corm criteria of saffron (Crocus sativus L.). J. Water Soil. 25, 196–206. [in Persian with English Summary].
Koocheki, A., Siahmarguee, A., Azizi, G., Jahani, M., and Alimoradi, L., 2009a. The effect of plant density and depth on agronomic characteristic of saffron (Crocus sativus L.) 3rd International symposium on saffron. Forthcoming challenges in cultivation, Research and Economics. 20-23 May. Korokos, Kozami, Greece.
Kumar, R., Singh, V., Devi, K., Sharma, M., Singh, M.K., andAhuja, P.S., 2009. State of art of saffron (Crocus sativus L.) agronomy: a comprehensive review. Food Rev. Int. 25, 44–85.
Lozano, P., Castellar, M.R., Simancas, M.J., and Iborra, J.L., 1999. Quantitative high performance liquid chromatographic method to analyse commercial saffron (Crocus sativus L.) products. J. Chromatogr. 830, 477–483.
Magesh, V., Singh, J.P.V., Selvendiran, K., Ekambaram, G., and Sakthisekaran, D., 2006. Antitumour activity of crocetin in accordance to tumor incidence, antioxidant status, drug metabolizing enzymes and histo pathological studies. Mol. Cell. Biochem. 287 (1–2), 127–135.
Mathew, B., 1999. Botany, taxonomy and cytology of C. sativus L. and its allies. In: Negbi, M., (Ed.), Saffron: Crocus sativus L. Harwood Academic Publishers, Amsterdam, pp. 19–30.
Mc-Gimpssey, G.A., Douglas, M.H., and Wallace, A.R., 1997. Evaluation of saffron (Crocus sativus L.) production in New Zealand. New Zealand J. Crop Hort. Sci. 25, 159-168.
Naderi-Darbaghshahi, M.R., Khajeh-Bashi, S.M., Bani-Ateba, S.A.R., and Deh-Dashti, S.M., 2008. Effects of planting method, density and depth on yield and exploitation period of saffron (Crocus sativus L.) in Isfahan. J. Seed Plant 24, 643-657. [in Persian with English Summary].
Rangahou, M.K., 2003. Growing saffron. The world's most expensive spice. Crop Food Res. 20, 1-4.
Renau-Morata, B., Nebauer, S.G., Sánchez, M., and Molina, R.V., 2012. Effect of corm size, water Stress and cultivation conditions on photosynthesis and biomass partitioning during the vegetative growth of saffron (Crocus sativus L.). Ind. Crop Prod. 39, 40–46.
SAS Institute., 2003. SAS/STAT User’s Guide, Version 9.1.SAS Institute, Cary, NC.
Tarantilis, P.A., and Polissiou, M., 1997. Isolation and identification of the aroma constituents of saffron (Crocus sativa L.). J. Agric. Food Chem. 45, 459–462.