Document Type : Original Article

Authors

1 Assistant Professor, Department of Horticultural Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

2 Associate professor, Department of Agronomy, Malekan Branch, Islamic Azad University, Malekan, Iran.

Abstract

Introduction: Saffron (Crocus sativus L.) is one of the most valuable medicinal plants globally, known for its culinary, medicinal, and economic importance. The yield and quality of saffron are influenced by several agronomic and environmental factors. Among these, nutrient management plays a critical role in enhancing plant growth and productivity. The use of organic compounds such as humic and folic acids has gained attention due to their potential to improve plant health, nutrient uptake, and stress resistance. Additionally, micronutrients like zinc are essential for various biochemical processes in plants, affecting overall growth, chlorophyll synthesis, and reproductive success. Recent studies suggest that the combined application of organic acids and micronutrients can synergistically enhance crop performance. This study investigates the effects of humic acid, folic acid, and zinc sulfate on key growth, physiological, and reproductive traits of saffron to identify the most effective treatment combinations for improving saffron yield and quality.
 
Materials and Methods: In this study, the studied treatments are humic acid (no use of humic acid, use of humic acid and use of humic acid enriched with amino acids), folic acid (no use of folic acid, use of folic acid and use of folic acid enriched with amino acids). amino) and zinc sulfate (no application of zinc sulfate, application of zinc sulfate in the amount of 3 kg/ha and application of zinc sulfate in the amount of 6 kg/ha) on growth, flower production and quality characteristics of saffron essential oil. This experiment was carried out in two years in a factorial manner based on a randomized complete block design in three replications in the fields of the Islamic Azad University of Malkan branch. The treatments were applied as soil application every two years after planting.
 
Results and discution: This study examined the effects of humic acid, folic acid, and zinc sulfate on saffron plant growth over two years. The results showed that the highest plant height, 14.33 cm and 14.2 cm, was recorded in the second year with the treatments of humic acid + folic acid + zinc sulfate at 6 kg/ha and humic acid enriched with amino acids + folic acid enriched with amino acids without zinc sulfate. Compared to untreated plants, these treatments increased height by 64% and 62%, respectively. Similar results were observed in other growth traits, with humic and folic acids enriched with amino acids, along with zinc sulfate, yielding the most positive effects, especially in the second year. The application of enriched humic acid increased leaf number by 28.3%, while regular humic acid resulted in a 12% increase. The dry weight of aerial parts showed no significant difference between the two treatments, but leaf number, surface area, and dry weight were all positively influenced by these treatments. Zinc sulfate also had a significant impact, increasing leaf surface area by 46% in the second year when combined with humic acid. The study highlighted that zinc sulfate at 6 kg/ha, enriched humic acid, and folic acid enriched with amino acids led to the highest chlorophyll content and improved nutrient uptake, especially nitrogen and phosphorus. Additionally, the treatments positively impacted the reproductive traits of saffron, such as flower number and stigma weight, with humic acid enriched with amino acids having the greatest effect. Folic acid, when enriched with amino acids, significantly increased crocin content, while both humic and folic acids improved picrocrocin levels. The study concludes that enriched humic and folic acids, combined with zinc sulfate, have the most substantial effects on saffron's vegetative and reproductive traits.
 
Conclusion: These results show that the combination of humic and folic acid treatments enriched with amino acids and zinc sulfate can effectively affect the growth and quality traits of saffron, especially the number of flowers and stigma weight.

Keywords

Abdul Majid, L., Saeed, K., Muhammad, M. 2023. Effect of nano fertilizers and amino acid of tryptophan on some physiological characters of saffron plant. Kirkuk University Journal. 5, 151-167.
Alam, N., Anis, M., Javed, S.B. (2020). Stimulatory effect of copper and zinc sulphate on plant regeneration, glutathione-S-transferase analysis and assessment of antioxidant activities in Mucuna pruriens L. (DC). Plant Cell Tiss Organ Cult, 141, 155–166.
Alaoui, I., Ghadraoui, O., Serbouti, S. (2022). The mechanism of absorption and nutrients transport in plants: A review. Tropical Journal of Natural Product Research, 6, 8-14.
Al-Maliky, A., Jerry, A., Obead, F. (2019). The effects of foliar spraying of folic acid and cysteine on growth, chemical composition of leaves and green yield of faba bean (Vicia faba L.). Basrah Journal of Agricultural Sciences, 32, 223-229.
 Alsamadany, H., Mansour, H., Elkelish, A., Ibrahim, M.F.M. (2022). Folic Acid Confers Tolerance against Salt Stress-Induced Oxidative Damages in Snap Beans through Regulation Growth, Metabolites, Antioxidant Machinery and Gene Expression. Plants, 11(11), 1459-1467.
Aminifard, M. H., Shakeri, M., Behdani, M. A., Tabatabaei, J. (2024). Effects of gibberellic acid and plant density on antioxidant activity and secondary metabolites of saffron (Crocus sativus L.). Journal of Saffron Research, 12, 51-65. (in Persian)
Armak, A., Feizi, H., Alipanah, M. (2018). Impact of use of different sources of humic, bio and nano fertilizers and nitrogen levels on saffron (Crocus sativus L.) flower yield. 10.22048/jsat.2017.61855.1193.
Baqer, H., Zeboon, N., Al-Behadili, A. (2019). The role and importance of amino acids within plants: a review. Plant Archives, 19, 1402-1410.
El-kosary, S., El-Shenawy, I. E., Radwin, S.I. 2011. Effect of microelements, amino acids on growth, flowering and fruiting of some mango cultivars. Journal of Horticultural and Ornamental plants, 3, 152-161.
Gorelova, V., Ambach, L., Rébeillé, F., Stove, C., Van Der Straeten, D. (2017). Folates in Plants: Research Advances and Progress in Crop Biofortification. Front Chem, 29, 21-28.
Haghighi, M., Barzegar Sadeghabad, A., Abolghasemi, R. (2022). Effect of exogenous amino acids application on the biochemical, antioxidant, and nutritional value of some leafy cabbage cultivars. Sci Rep, 12, 65-73.
Hourani, G. (2023). Effect of fertilizers on growth and productivity of saffron: A review. Agronomy research. 21, 87-105.
Xu, J., Mohamed, E., Li, Q., Lu, T., Yu, H. and Jiang, W. (2021). Effect of humic acid addition on buffering capacity and nutrient storage capacity of soilless substrates. Front. Plant Sci, 12, 56-63.
INS (Iran National Standard). (2006). Research Institute of Standard and Iran. Saffron Bulletin, 259, 124-133.
Jung, S., Rickert, D. A., Deak, N. A., Aldin, E. D., Recknor, J., Johnson, L. A. and Murphy, P. A. (2003). Comparison of kjeldahl and dumas Methods for determining protein contents of soybean products. Journal of the American Oil Chemists Society, 8(12), 1169-1173.
Kauser, A., Malik, S., Azam, G. (1985). Effect of humic acid on wheat (Triticum aestivum L.) seedling growth. Environmental and Experimental Botany, 25, 245-252.
Kwame, A., Malinda, S., Linda Yuya, G. (2022). Understanding the role of humic acids on crop performance and soil health. Frontiers in Agronomy, 4, 78-98.
Li, Y., Fang, F., Wei, J. (2019). Humic acid fertilizer improved soil properties and soil microbial diversity of continuous cropping peanut: a three-year experiment. Sci Rep, 9, 12014.
Li, Y., J. Luo, R. Chen, Y. Zhou, H. Yu, Z. Chu, Y. Lu, X. Gu, S. Wu, P. Wang, H. Kuang, Bo Ouyang. (2023). Folate shapes plant root architecture by affecting auxin distribution. The Plant Journal, 5, 123-132.
Moghaddam, S.M. (2024). The effect of corm storage conditions during the summer dormancy stage on reproductive growth and yield of saffron. Journal of Saffron Research, 12, 1-14. (in Persian)
Nargesi, M., Sedaghathoor, G. and Hashemabadi, D. (2022). Effect of foliar application of amino acid, humic acid and fulvic acid on the oil content and quality of olive. Saudi J Biol Sci, 29(5), 3473–3481.
Nouri, A.R., Koocheki, A. R., and Nassiri, M. (2024). Effects of mycorrhiza inoculation, mother corm weight and humic acid on daughter corm and flower yield of saffron. Journal of Saffron Research, 11, 48-65. (in Persian)
Psarras, G., Manolikaki, I., Dareioti, M., Digalaki, N., Sergentani, C., Barbopoulou, E., Koubouris, G. (2024). Effect of amino acids application on flowering, vegetation, yield, and oil of olive (Olea europaea L.) variety ‘Koroneiki.’ Journal of Plant Nutrition, 47(13), 2057–2069.
Rademacher W. (1994). Gibberellin formation in microorganisms. Plant Growth Regul, 15,  303-314.
Rossi, L., Fedenia, L., Sharifan, H., Ma, X., Lombardini, L. (2019). Effects of foliar application of zinc sulfate and zinc nanoparticles in coffee (Coffea arabica L.) plants. Plant Physiol Biochem, 135, 160-166.
Sadeghi Chah-Nasir, A., Abootalebi Jahromi, A., Behrooznam, B., Hassanzadeh Khankahdani, H., Ejraei, A. (2023). Effect of humic acid and amino acid foliar applications on the growth characteristics, yield, and fruit quality of tomato (Solanum lycopersicom L.). International Journal of Horticultural Science and Technology, 10(3), 309-318.
Shahin M.F.M., Genaidy, E. and Haggag, L. (2015). Impact of Amino Acids, Vinasse and Humic Acid as Soil Application on Fruit Quality and Quantaty of " Kalamata " Olive Trees. International Journal of ChemTech Research, 8, 75-84.
Shahrajabian, M. H., Cheng, Q., Sun, W. (2022). The effects of amino acids, phenols and protein hydrolysates as biostimulants on sustainable crop production and alleviated stress. Recent Pat Biotechnol, 16(4), 319-328.
Tuan, H. (2014). A review of plants' flowering physiology: the control of floral induction by juvenility, temperature and photoperiod in annual and ornamental crops. Asian Journal of Agriculture and Food Science, 2, 186-195.
Turkmen, N., Sari, F., and Veliglu, Y.S. (2005). The effect of cooking methods on total phenolic and antioxidant activity of selected green vegetables, Food Chemistry, 93, 713- 718.
Umair, M., Huma Zafar, S., Cheema, M., Minhas, R., Manan Saeed, A., Saqib, M. and Aslam, M. (2023). Unraveling the effects of zinc sulfate nanoparticles and potassium fertilizers on quality of maize and associated health risks in Cd contaminated soils under different moisture regimes. Science of the Total Environment, 7, 150-164.
Wang, S., Li, X., Zhang, M., Jiang, H. and Li, M. (2022). Dietary supplementation of crystalline amino acid improves growth performance and health of yellow catfish that reduced by plant proteins replacement of fishmeal. Hidawi.
Westerman, R. E. L. (1990). Soil testing and plant analysis. Soil Science Society of America.
Zayed, B. (2020). Folic acid as foliar application can improve growth and yield characters of rice plants under irrigation with drainage water. Fresenius Environmental Bulletin, 29, 9420-9428.