Effects of Irrigation Water Quality, Bio-fertilizer and Nanoparticles of Fe on Yield and Some Physiological Traits of Saffron (Crocus sativus L.)

Salariyan, Alijan; Mahmoodi, Sohrab; Behdani, Mohammad Ali; Kaveh, Hamed

doi:10.22077/jsr.2021.4471.1164

Document Type : Original Article

Authors

¹ PhD Graduated Student in Agronomy of University of Birjand, Researcher of Saffron Institute, University of Torbat Heydarieh, Torbat Heydarieh, Iran.

² Associate Professor, Department of Plant Production and Genetics, Academic Staff of Saffron Research Group and Plant and Environmental Stresses Research Group, Faculty of Agriculture, University of Birjand, Birjand, Iran.

³ Professor, Department of Plant Production and Genetics, Academic Staff of Saffron Research Group and Plant and Environmental Stresses Research Group, Faculty of Agriculture, University of Birjand, Birjand, Iran.

⁴ Assistant Professor, Department of Plant Production, Faculty of Agriculture, University of Torbat Heydarieh, Torbat Heydarieh, Iran.

https://doi.org/10.22077/jsr.2021.4471.1164

Abstract

Introduction: Nutrition management in saffron cultivation is one of the important issues in the production of this valuable product. Studies have shown that the use of bio-fertilizers and nano-fertilizers can be effective in reducing the inhibitory effects of drought and salinity stress. Also nano-fertilizers have high consumption efficiency and can optimally release their nutrients at a suitable point in the root growth area. The tendency of saffron to grow in a dry climate has caused the cultivation of this plant to expand in the eastern lands of the country. Meanwhile, the lands in the east of the country often face drought and salinity problems due to low and scattered rainfall. Thus, present study was performed to investigate the effects of salinity levels, bio-fertilizer and nano-fertilizer of Fe on yield and physiological characteristics of saffron.

Materials and Methods: Present study was performed in a farm that located in Torbat Heydarieh city, in 2018-2019. The experiment was conducted as factorial layout based on a randomized complete block design with three replications and analyzed by combined analysis in location (salinity). Bio-fertilizer was applied at four levels of zero, 500, 1000 and 1500 kg ha^-1 and nano particles of Fe was applied at two levels of non-used and application of four liters per hectare. These factors were investigated in two locations with different irrigation salinity (2.29 and 4.49 dS m^-1).

Results and Discussion: The results showed that there was the highest value for dry weight of stigma in irrigation conditions with salinity of 2.29 dS m^-1. In this trait, under the conditions of irrigation with salinity of 2.29 dS m^-1, there was no significant difference between the application and non-application levels of nano-fertilizer of Fe; However, in the conditions of irrigation with salinity of 4.49 dS m^-1, the application of nano-fertilizer of Fe compared to non-application, caused a significant increase of 32.8% for dry weight of stigma. Comparison of mean for triple interaction showed that in irrigation conditions with salinity of 4.49 dS m^-1 and in all levels of bio-fertilizer, the application of nano-fertilizer of Fe compared to control increased the amount of chlorophyll b and RWC, significantly. At both salinity levels of 2.29 and 4.49 dS m^-1, by increasing amounts of bio-fertilizer, the amount of carotenoids significantly increased. Also, at both salinity levels of 2.29 and 4.49 dS m^-1 and all levels of bio-fertilizer, the application of Fe nano-fertilizer significantly increased carotenoids compared to conditions of non-application. In irrigation conditions with salinity of 4.49 dS m^-1 and at levels of zero and 500 kg ha^-1 of bio-fertilizer, the application of Fe nano-fertilizer prevented increasing of proline, significantly and prevented the occurrence of more electrolyte leakage.

Conclusion: In the present research, increasing the salinity from 2.29 to 4.49 dS m^-1 caused a significant decrease in dry weight of saffron stigma. However, in the conditions of applying irrigation with a salinity of 4.49 dS m^-1, the application of iron nano-fertilizer caused a significant increase in dry weight of stigma compared to its non-application. With increasing amounts of bio-fertilizer in both irrigation conditions, the amount of chlorophyll a also increased. In addition, in irrigation conditions with a salinity of 49.4 dS m^-1 and at all levels of bio-fertilizer, the application of iron nano-fertilizer significantly increased the amount of chlorophyll b and RWC, compared to the conditions without its application. Also, in irrigation conditions with salinity of 4.49 dS m^-1 and at the levels of zero and 500 kg ha^-1 of bio-fertilizer, the application of iron nano-fertilizer significantly prevented the increase of proline and in the same conditions and at all levels of bio-fertilizer, it prevented to do more electrolytes leakage. Based on these results, in order to achieve higher yields in saffron cultivation, irrigation with less saline water is recommended and in case of irrigation with salinity of more than 4.2 dS m^-1 in saffron cultivation, the use of iron and bio-fertilizers is recommended to improve the physiological characteristics.

Keywords

20.1001.1.23453869.1402.11.1.3.4

References

Adolf, V.I., Jacobsen, S.E., & Shabala, S. (2013). Salt tolerance mechanisms in quinoa (Chenopodium quinoa Willd.). Environmental and Experimental Botany, 92, 43-54.

Arshadi, M.J. (2016). Investigation of the effect of seeds inoculation of chickpea (Cicer arietinium L.) with arbuscular mycorrhiza and pseudo-endomycorrhiza in response to drought stress (Ph.D thesis), Ferdowsi University of Mashhad. Mashhad, Iran. [in Persian].

Asghari, R., Dadashi, M., Razavi, A., Feizi, H., & Bakhtiari S. (2019). Effect of cow manure on yield and morphological and physiological characteristics of saffron (Crocus sativus L.) under salinity stress. Saffron Agronomy and Technology 7(2), 171-184. [in Persian].

Baghaee, N.,& Maleki Farahani, S. (2013). Comparison of chelate fertilizer of Fe with nano and micro bases on quantitative yield and allocation of photosynthetic materials of saffron (Crocus sativus L.). Saffron Agronomy and Technology 1(2), 156-169. [in Persian].

Bates, I.S., Waldern, R.P., & Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil 39, 205-207.

Blum, A., Klueva, N., & Nguyen, H.T. (2001). Wheat cellular thermos-tolerance is related to yield under stress. Euphytica 117, 117-123.

Cha-um, S., Batin, C.B., Samphumphung, T., & Kidmanee, C. (2013). Physio-morphological changes of cowpea (Vigna unguiculata Walp.) and jack bean (Canavalia ensiformis L.) in responses to soil salinity. Austrian Journal of Crop Science 7(13), 2128–2135.

Chimenti, C.A., Marcantonio, M., & Hall, A.J., (2006). Divergent selection for osmotic adjustment results in improved drought tolerance in maize (Zea mays L.) in both early growth and flowering phases. Field Crops Research 95, 305-315.

Cui, H., Sun, C., Liu, Q., Jiang, J., & Gu, W. (2006). Applications of nanotechnology in agrochemical formulation, perspectives, challenges and strategies. institute of environment and sustainable development in agriculture. Chinese Academy of agricultural sciences, Beijing, China.

El Sabagh, A., Hossain, A., Barutcular, C., Gormus, O., Ahmad, Z., Hussain, S., Islam, M., Alharby, H., Bamagoos, A., Kumar, N., Akdeniz, H., Fahad, S., Meena, R.S., Abdelhamid, M., Wasaya, A., Hasanuzzaman, M., Sorour, S., & Saneoka, H. (2019). Effects of drought stress on the quality of major oilseed crops: Implications and possible mitigation strategies, Applied Ecology and Environmental Research, 17,4019-4043.

Erfaniyan, M., Vesal, S.R., & Bagheri, A.R. (2014). Trends in morpho-physiological traits of chickpea (Cicer arietinum L.) genotypes in response to salinity stress induced by sodium chloride. Iranian Journal of Pulses Research 5(1), 117-128. [in Persian].

Garg, N., & Singla, R. (2009). Variability in the response of chickpea cultivars to short-term salinity, in terms of water retention capacity, membrane permeability and osmo-protection. Turkish Journal of Agriculture and Foresty, 33, 1-7.

Haji Nia, S., Zare, M.J., Mohammadi Gol Tappeh, E., & Rejali, F. (2011). Evaluation of the usefulness of Piriformospora indica endophytic fungus and Azospirillum Sp in increasing the tolerance of wheat of Sardari cultivar (Triticum aestivum) to salinity stress. Environmental stresses in agricultural sciences, 4(1), 21-31. [in Persian].

Hessini, K., Issaoui, K., Ferchichi, S., Saif, T., Abdelly, C., Siddique, K.H.M., & Cristina, C. (2019). Interactive effects of salinity and nitrogen forms on plant growth, photosynthesis and osmotic adjustment in maize. International Journal of Plant Physiology and Biochemistry 139, 171-178.

Javadipoor, Z., Movahedi Dehnavi, M., & Baluchi, H.R. (2013). Evaluation of photosynthetic parameters, content and leaf fluorescence of safflower cultivars under salinity stress. Electronic Journal of Crop Production 6(2), 35-56. [in Persian].

Kafi, M., Borzuie, A., Salehi, M., Kamandi, M., Masumi, A., & Nabati, J. (2018). Physiology of Environmental Stresses in Plants. Jihad Daneshgahi Publications of Mashhad. 502 p. [in Persian].

Kafi, M., Hemati Kakhki, A., & Karbasi, A. (2002). Saffron, production and processing technology. Ferdowsi University of Mashhad Publications. 276 p. [in Persian].

Khosravi, H., Samar, S.M., Fallahi, E., Davoodi, H., & Shahabian, M. (2009). Inoculation of Golden Delicious' apple trees on M9 root stock with Azotobacter improves nutrient uptake and growth indices. Journal of Plant Nutrition 32, 946-953.

Kirmani, N.A., Sofi, J.A., Bhat, M.A., & Ansar-Ul-Haq, S. (2014). Sustainable saffron production as influenced by integrated nitrogen management in typic hapludalfs of NW Himalayas. Communications in Soil Science and Plant Analysis 45, 653-668.

Koocheki, A.R., & Sarmad Nia, Gh. (2013). Crop physiology. Ferdowsi University of Mashhad Publications. [In Persian].

Lai, R., (2007). Soil science in the Era of hydrogen economy and 10 billion people. The Ohio state University, USA. pp. 1-9.

Mansoorifar, S., Shaban, M., Ghobadi, M., & Sabaghpoor, S.H. (2012). Physiological characteristics of chickpea (cicer arietinum L.) cultivars under drought stress and nitrogen fertilizer as starter. Iranian Journal of Pulses Research 3(1), 101-110.[in Persian].

Naghizadeh, M., Gholami Shabestari, M., & Shamsaddin Saied, M. (2014). The study of some physiological responses of three Iranian saffron (Crocus sativus L.) landraces to salinity stress. Saffron Agronomy and Technology 2(3), 127-136. [in Persian].

Paivandi, M., Parande, H., & Mirza, M. (2011). compared the impact of Nano iron chelated with iron chelate on growth parameters and antioxidant enzyme activity of Ocimum Basilicum. Journal of Cellular and Molecular Biology. 4, 1-12.

Rezaei Chiyaneh, E. Zehtab Salmasi, S. Ghassemi Golezani, K. & delazar, A. (2013). Physiological responses of fennel (Foeniculum vulgare L.) to water limitation. Journal of Agroecology 4(4), 347-355. [in Persian].

Rezazi, A., Labafi, M.R., Mehrabi, Z., Nazeran, M.H., & Khalaj, H. (2010). Effect of iron chelate nano fertilizer on yield of saffron (Crocus sativus L.). 11^th Iranian Congress of Agricultural Sciences and Plant Breeding. 2^nd to 4^th of August 2010, Shahid Beheshti University, Iran. [in Persian].

Ritchie, S.W., & Nguyen, H.T. (1990). Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science, 30, 105-111.

Rostami, G., Moghaddam, M., Ghasemi, A., & Tehranifar, A. (2018). Effect of foliar application of iron and zinc to sulfated and nanoparticle forms on morphological and biochemical properties of peppermint under salinity stress. Journal of Environmental Stresses in Crop Sciences. 11(3), 707- 720. [in Persian].

Rostami, M., Mohammad Parast, B., & Golfam, R. (2015). Effect of different levels of salinity stress on some physiological characteristics of saffron (Crocus sativus L.). Saffron Agronomy and Technology. 3(3), 179-193. [in Persian].

Sahabi, H. (2017). Effect of maternal corm weight and foliar application on yield and characteristics of daughter corm of Iranian and Spanish saffron (Crocus sativus L.) corms. (Master’s thesis). Faculty of Agriculture, Ferdowsi University of Mashhad. [in Persian].

Seyed Sharifi, R., & Seyed Sharifi, R. (2019). The effect of bio-fertilizer application on yield, oil content and antioxidant activity of sunflower enzymes in cut-off irrigation treatments. Journal of Plant Process and Function. 8, 97-107. [in Persian].

Shinde, S., Villamor, J.G., Lin, W., Sharma, S., & Verslues, P.E. (2016). Proline coordination with fatty acid synthesis and redox metabolism of chloroplast and mitochondria. Journal of Plant Physiology. 172, 1074-1088.

Taiz, L., & Zeiger, E. (2003). Plant Physiology. Sinauer Associates, Inc. Publishers.

Wellburn, A.R. (1994). The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology. 144, 307-313.

Yamada, M., Morishita, H., Urano, K., Shiozaki, N., Yamaguchi Shinozaki, K., Shinozaki, K., & Yoshiba, Y. (2005). Effects of free proline accumulation in petunias under drought stress. Journal of Experimental Botany. 56, 1975-1981.

Zahedi, H., & Alipoor, A. (2018). Effect of foliar application of iron and manganese nano chelate on yield and yield components of barley (Hordeum Vulgare L.) under dehydration stress conditions at different growth stages. Journal of Environmental Stresses in Crop Sciences. 11(4), 861- 647. [in Persian].