Molecular Detection and Investigation of Irish Severe Mosaic Virus in Saffron, Fields of Razavi and South Khorasan

Tavoosi, Masume

doi:10.22077/jsr.2021.4239.1157

Document Type : Original Article

Author

Masume Tavoosi

Faculty of Agriculture

https://doi.org/10.22077/jsr.2021.4239.1157

Abstract

Saffron (Crocus sativus) is related to Iridaceae family and is one of the most important products in Iran. Recently, different viral diseases were detected on it. Iris severe mosaic virus is detected on some ornamental plants in Iran but has not been reported on saffron. In order to detect this virus, we collected 130 samples with yellowing, stunning, mosaic symptoms on saffron leaves and they were tested by PCR and specific primers. Finally fragments with 917 bp were amplified in 5 samples. Samples were cloned or more investigations in Macrogene company of Korea. Investigation of phylogeny according to coat protein showed that 5 Iranian isolates were separated in two groups near to Chinese isolates. Investigation in this survey showed that different groups were separated according their geographical distribution. Recombinant investigation showed that in none of Iranian isolates there is no recombination. This research is the first report of this virus and first molecular and phylogeny investigation in saffron fields of South and Razavi Khorasan.

Keywords

References

Abrishami, M.H. 2004. Saffron: from the past to the present. Encyclopedia of production, trade and use. Amirkabir Publishing Co. (In Persian with English summary).

Ahmadia, A. 2013. Important medicinal plants of Iran. Torbat Heydariyeh University Press, 169- 173. (In Persian).

Caiola, M. G., and Canin, A. 2010. Looking for saffron (Crocus sativus L.) Parent's.Functional Plant Science and Biotechnology. Global Science Books.

Ebrahimzade, H., Rajabian, T., Karimian, R., and Abrishami, P. 2006. Iranian saffron: with a research look. Etelaat publisher. (In Persian with English summary).

Ghorbanpoor, M., Yuosefy Javan, I. 2016. A review of medicinal properties and side effects of saffron (Crocus sativus L.), The first conference on aromatic and spicy herbs, Gonbad Kavus university. (In Persian).

Farokhvand, S., Hosseini, S. A & Aminifard, M. H. 2019. Serological and molecular detction of Tomato spotted wilt virus in some weed of saffron fields. Journal of saffron research., 6(2):167-177. (In Persian).

Heydari, M., Hosseini, A. & Duri, R. (2018). Detection and Phylogenetic Analysis of Turnip mosaic virus of Saffron (Crocus sativus) in Iran. App.Res. in Plant Protec, 17-28. (In Persian).

http://www.ncbi.nlm.nih

Heydari, M., Hosseini,S. A. & Duri, R. 2018. Serological and molecular detection of TuMV on Globachia laevigata in saffron fields. Journal of saffron research., 5(2):191-200. (In Persian).

Inouye N, Mitsuhata K (1978) Turnip mosaic virus isolated from Iris. Nogaku Kenkyu, 57, 1–16(in Japanese).

Karbassi, A. 2016. Economic and social analysis of saffron cultivation in Torbat Heydariyeh city. 2th conference of perspective of Torbat Heydariyeh region on the horizon in 1404, Torbat Heydarie, Iran. (In Persian).

koochaki, A., Tabrizi, L., Jahani, M., & Mohammad Abadi, A. A. (2012). An evaluation of the effect of saffron (CrocussativusL.) corm planting rate and pattern on the crop's performance. Iranian J of Hortic. Scie. 42, 379-391. (In Persian).

Kulshrestha S, Hallan V, Raikhy G, Kumar A, Ram R, Garg ID,Zaidi AA (2004) Molecular characterization of an Iris severe mosaic virus isolate from India. Acta Virol, 48, 65–67.

Li Y, Deng C, Shang Q, Zhao X, Liu X, Zhou Q. 2016. The first complete genome sequence of Iris severe mosaic virus. Arch Virol, 161, 1069 –1072.

Muhire,B. M., Varsani, A. & Martin, D. P. (2014). SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PloS one, 9,1-8.

Muhtashami, T. 2018. Factors Affecting Excessive Nitrogen Fertilizer Use in Saffron Cultivation: (Case Study: Torbat Heydarieh County). Journal of saffron research., 6(1):127-140. (In Persian).

Nateqi, M., Habibi, M. K., Dizadji, A., & Parizad, S. (2015). Detection and molecular characterization of the Iris severe mosaic virus-Ir isolate from Iran. J. Plant Protet Res. 11(2), 22-30.

Nishigawa, N., Tukoda, R., Yushida, T, et al. (2021). Complete genome sequence of Iris severe mosaic virus isolsted in Japan. Microbiol, 8 (15): 3-19.

Park WM, Lee SS, Park SH, Ryu KH (2000) Sequence analysis ofthe coat protein gene of a Korean isolate of Iris severe mosaic virus from Iris plant. Plant Pathol, 6, 36–42.

Pearson, N., Cohen, P., Covel, S.J, et al. 2009. A survey of viruses of flower bulbs in New Zealand. Australian Plant Pathol, 38, 305-309.

Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. (2013). MEGA X: molecular evolutionary genetics analysis version 6.0. Mol. Boil. Evol., 30, 2725-2729.

Thompson, J. D., Higgins, D. G. & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids res, 22, 4673-4680.

Van der Vlugt C.I.M., Langeveld S.A., Goldbach R.W. 1994. Molecular cloning and sequence analysis of 3’-terminal region of Iris severe mosaic virus RNA. Arch of Virol, 136, 397–406.

Winterhalter, P., and Stranbinger, M. 2000. Saffron renewed interests in an ancient spice. Food Rev. Int, 39-59.

Yan SJ, Qin ZD, Jin LL, Chen JS (2010) A new isolate of Iris severe mosaic virus causing yellow mosaic in Iris ensata Thunb.J Nanosci Nanotechnol, 726–730.