Induced Morpho-physiological Variation in Saffron (Crocus sativus L.) Using Gamma Radiation

Seifati, S. Ebrahim; Mohit Ardakani, Ali Mohammad; Izanloo, Ali; Borzoei, Azam

doi:10.22077/jsr.2020.3742.1140

Document Type : Original Article

Authors

¹ Yazd University, Yazd, IRAN

² Department of Agronomy and Plant breeding

³ Nuclear Science and Technology Research Institute

https://doi.org/10.22077/jsr.2020.3742.1140

Abstract

In order to investigate the diversity of irradiated genotypes at two levels of 15 and 18 Gy gamma ray with control genotype (without radiation), an experiment was conducted in a completely randomized design in the greenhouse of Yazd University. The results of analysis of variance showed that the first generation mutants and control genotype were significantly different in terms of eight morphological characteristics and total carotenoid content. While there was no significant difference among them based on the number of stems, number of flowers as well as chlorophyll and leaf proline content. Based on the results of cluster analysis, the studied genotypes were divided into two groups and the accuracy of grouping based on the detection function was 93%. The first group consisted of 24 18-Gy mutants and a control genotype. All of 15 Gy mutants, 6 mutants of 18 Gy and 10 control genotypes were in the second group. Based on the graphical representation of biplot analysis method, 7 mutants at 18 Gy dose and 5 mutants in 15 Gy radiation dose were located in polygonal vertices, which showed the greatest difference between them and genotype without radiation. Accordingly, traits related to plant yield and reproductive growth had the greatest effect in determining superior mutations. According to the aim of this study, 15 Gy dose of gamma radiation resulted in mutations with better performance and 18 Gy dose caused more morpho-physiological diversity in saffron stems.

Keywords

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